|
|
 |
基因轉(zhuǎn)移技術(shù)國際技術(shù)講座
in-vivo Electroporation Seminars
|
|
 |
為推廣體內(nèi)局部基因轉(zhuǎn)移技術(shù)、交流國際間相關(guān)技術(shù)的發(fā)展現(xiàn)狀，擬定于2002年9月份在上海、北京和廣州等三地分別舉辦EP技術(shù)交流會，特邀請國際著名專家日本名古屋大學(xué)村松達夫教授主講。具體時間、地點如下。歡迎相關(guān)專業(yè)的學(xué)者和老師前來指導(dǎo)和與專家交流。
|
時間地點：
|
9月16日：上海第二醫(yī)科大學(xué) |
| 9月18日：北京大學(xué)醫(yī)學(xué)部學(xué)術(shù)會議中心 |
| 9月20日：廣州中山大學(xué)中山醫(yī)學(xué)院。 |
講座內(nèi)容：
|
There are serious concerns about the use of viral vectors for in vivo gene transfer, especially gene therapy, such as oncogenic potential and high immunogenicity. Accordingly, an easy, safe, non-toxic and possibly efficient delivery means of genes to a specified target tissue has been an attractive issue for researchers. In this sense, in vivo electroporation (EP) is thought to be one of the promising approaches since : handling is simple, quick, i.e., within a matter of seconds, and easy; transfection efficiency is probably higher than any other nonviral method; immunogenicity is low; there may be no limitation in size and concentration of DNA transferred, and ; no special preparation of DNA is required. Although gene expression exerted is transient when transfected by in vivo EP, foreign gene expression may last as long as more than 1 year.
In EP procedures, tissues of living animals are subjected to electric field that causes not only membrane pores but also shape deformation of cells, leading to the induced membrane permeability, hence transport of large molecules such as DNA across the cell membrane. To my best knowledge, no extensive and systemic analyses of EP parameters have been conducted so far, and therefore, most experimental conditions have been empirically known. Nevertheless, the results obtained are implicative that in vivo gene EP could be applied to a wide variety of experimental and therapeutic purposes. In this brief talk, some examples are presented in relation to gene therapy, growth promotion, pharmaceutical protein production and embryo manipulation. |
聯(lián)系方式：
|
北京：電話：(10) 51659588 聯(lián)系人：王征星 |
| 上海：電話：(21) 62891803 聯(lián)系人：沈建英 |
| 廣州：電話：(20) 83229830 聯(lián)系人：楊曉新 |
|
|
