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實(shí)驗(yàn)室超濾-Vivapure離子交換蛋白純化濃縮器產(chǎn)品

Vivascience Vivapure Ion Exchange System for Protein Purifications

Vivapure離子交換膜蛋白純化濃縮器

從混合物中分離和純化蛋白質(zhì)，是生物制藥和其他生物工程研究的重要步驟。目前應(yīng)用最為廣泛的方法是離子交換色譜法，但這種方法所需時(shí)間長(zhǎng)，且費(fèi)用昂貴。Sartorius公司的Vivapure產(chǎn)品，為蛋白質(zhì)的分離和純化提供了全新的思路。離子交換層析是分離純化蛋白最常用的方法，Vivapure也采用了這一原理，在很多蛋白質(zhì)研究領(lǐng)域中可替代傳統(tǒng)的HPLC和FPLC法，并克服了傳統(tǒng)方法費(fèi)時(shí)長(zhǎng)、操作復(fù)雜、設(shè)備昂貴等不足。只需不到30分鐘，就能完成蛋白樣品的吸附、清洗和洗脫全過程，得到高純度的蛋白質(zhì)。

Vivapure centrifugal ion exchange membrane spin columns will dramatically change the way you isolate and purify proteins. Vivapure replaces time-consuming, tedious and expensive chromatographic methods for many protein applications. The rapid purification protocol based on Membrane Adsorbers allows the parallel separation of proteins with high yield in less than 20 minutes.

Ion exchange chromatography is among the most widely used methods for fractionation and purification of biological substances. Vivapure employs an innovative and powerful ion exchange membrane technology for separating proteins. The purification protocol involves only a few steps, making the isolation of your target protein from samples almost as simple as filtration.

Vivapure spin columns are available with strong or weak cation or anion charged membrane matrices. With these ion exchange membrane devices, protein binding, elution and consequent purification and concentration are made almost as simple as filtration.

The protein purified using the Vivapure spin column may be used in many downstream applications, e.g. sample fraction for 1D and 2D, SDS-PAGE, X-ray crystallization, NMR spectroscopy and related proteomics applications

Vivapure離子交換膜蛋白純化濃縮器的典型性能

Vivapure離子交換膜蛋白純化濃縮器可提供的膜類型

Vivapure離子交換膜蛋白純化濃縮器的容量和規(guī)格

Vivapure離子交換膜蛋白純化濃縮器的應(yīng)用

• Fractionation of protein mixtures prior to 2D-PAGE
• Sample preparation prior to 1-D or 2-D PAGE
• Removal of endotoxins from monoclonal antibodies
• Preparation of heme moiety from heme containing protein for functional analysis
• Polishing of His-tagged proteins
• General protein purification
• Detergent removal from protein solutions
• Purification of antibodies from serum, ascites or tissue culture supernatant
• Establishing a purification/prepurification protocol for a given protein
• HPLC/FPLC sample preparation
• Purification of membrane-bound proteins

Vivapure離子交換膜蛋白純化濃縮器的化學(xué)兼容性

Vivapure離子交換膜蛋白純化濃縮器產(chǎn)品選擇