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YMC-生物大分子分離液相色譜柱
YMC Bio-Macro Molecular Separation Columns
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凝膠色譜柱 |
型號(hào)和規(guī)格 |
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DIOL-GFC凝膠色譜柱應(yīng)用實(shí)例
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采用不同孔徑的色譜柱填料對(duì)蛋白質(zhì)的分離校正曲線
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1. Glutamate
dehydrogenase (谷氨酸脫氫酶)
2. Lactate dehydrogenase (乳酸脫氫酶)
3. Enolase (烯醇化酶)
4. Adenylate kinase (腺苷酸激酶)
5. Cytochrome C (細(xì)胞色素C) |
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Column |
YMC-Pack Diol
500 x 8.0 mmI.D. |
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Eluent |
0.1M KH2PO4
- K2HPO4 (pH 7.0) containing 0.2M NaCl |
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Flow rate |
0.7 mL/min |
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Temp |
ambient |
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Detection |
UV at 280 nm |
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蛋白質(zhì)的分離(分子量范圍：10,000 ~ X00,000)
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Column |
YMC-Pack Diol, 500x8.0mm |
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Eluent |
KH2PO4 - K2HPO4 (pH 7.0) containing 0.2M NaCl |
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Flow rate |
0.7 mL/min |
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Temp |
ambient |
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Detection |
UV at 280 nm |
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1. Glutamate
dehydrogenase (谷氨酸脫氫酶)
2. Lactate dehydrogenase (乳酸脫氫酶)
3. Enolase (烯醇化酶)
4. Adenylate kinase (腺苷酸激酶)
5. Cytochrome C (細(xì)胞色素C) |
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多肽的分離 (分子量范圍：≥10,000)
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1.Insulin
(牛胰島素)
2.Neurotensin (神經(jīng)降壓素)
3.Angiotensn-Ⅱ (血管緊張素Ⅱ)
4.Glycine (甘氨酸) |
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Column |
YMC-Pack
Diol 500 x 8.0 mmI.D. |
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Eluent |
0.1M
KH2PO4-K2HPO4 (pH7.0) NaCl/acetonitrile (70/30) |
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Flow rate |
0.7 mL/min |
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Temp |
ambient |
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Detection |
UV at
215 nm |
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Compounds |
MW |
化合物名稱 |
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1. Lysozyme |
14,400 |
溶菌酶 |
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2. Ribonuclease A |
13,700 |
核糖核酸酶 A |
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3. Cytochrome C |
12,400 |
細(xì)胞色素C |
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4. Insulin (Bovine) |
5,700 |
牛胰島素 |
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5. Insulin B chain |
3,496 |
胰島素B鏈 |
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6. α-Mating factor |
1,684 |
α-配位因子 |
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7. Neurotensin |
1,672 |
神經(jīng)降壓素 |
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8. Angiotensin-I |
1,296 |
血管緊張素Ⅰ |
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9. CCK-octapeptide |
1,143 |
CCK-八肽 |
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10. Bradikinin |
1,060 |
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11. Angiotensin-II |
1,040 |
血管緊張素Ⅱ |
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12. Oxytocin |
1,007 |
催產(chǎn)素 |
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13. Angiotensin-III |
931 |
血管緊張素Ⅲ |
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14. Met-enkephalin |
573 |
蛋氨酸-腦啡肽 |
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15. Leu-enkephalin |
555 |
亮氨酸-腦啡肽 |
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16. Tetraglycine |
246 |
四甘氨酸 |
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17. Glycine |
75 |
甘氨酸 |
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天然糖的分離和分子量測定
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對(duì)于分離和測定天然糖和水溶性大分子量化合物，可以分別單獨(dú)采用Diol-60，Diol-120，Diol-200或Diol-300型的色譜柱，也可采用不同的分子量范圍組合來進(jìn)行。
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Column |
YMC-Pack
Diol 500 x 8.0 mmI.D. |
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Eluent |
Water |
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Flow rate |
1.0
mL/min |
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Temp |
ambient |
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Detection |
RI |
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Compounds |
MW |
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1. Pullulan (P-800) |
853,000 |
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2. Pullulan (P-400) |
380,000 |
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3. Pullulan (P-200) |
186,000 |
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4. Pullulan (P-100) |
100,000 |
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5. Pullulan (P-50) |
48,000 |
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6. Pullulan (P-20) |
23,700 |
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7. Pullulan (P-10) |
12,200 |
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8. Pullulan (P-5) |
5,800 |
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9. maltopentadecaose (G15) |
2,448 |
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10. maltoundecaose (G11) |
1,800 |
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11. maltoheptaose (G7) |
1,152 |
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12. maltopentaose (G5) |
824 |
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13. maltopentriose (G3) |
504 |
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14. maltose (G2) |
342 |
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15. Glucose (G1) |
180 |
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上次更新時(shí)間：2010年06月07日
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