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YMC-生物大分子分離液相色譜柱
YMC Bio-Macro Molecular Separation Columns
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大孔徑柱 |
型號和規(guī)格 |
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YMC-Pack 大孔徑柱的應(yīng)用實例 (Wide-Pore Columns)
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寡聚核苷酸分析
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1. c-Ha-ras/12 (20mer); GTGGGCGCC-AGC-GGTGTGGG Ser
2.c-Ha-ras/12 (20mer); GTGGGCGCC-GCC-GGTGTGGG Ala
3. c-Ha-ras/12 (20mer); GTGGGCGCC-GAC-GGTGTGGG Asp
4. c-Ha-ras/12 (20mer); GTGGGCGCC-GTC-GGTGTGGG Val
5. c-Ha-ras/12 (20mer); GTGGGCGCC-CGC-GGTGTGGG Arg |
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Column |
YMC-Pack ODS-AP
(5μm, 300A) 150x4.6mmI.D. |
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Eluent |
A) 100mM 三乙胺/乙酸(pH6.5)/乙腈(94/6)
B) 100mM 三乙胺/乙酸(pH6.5)/乙腈(85/15)
0-100%B (0-25min, linear) |
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Flow rate |
1.0 mL/min |
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Temp |
30℃ |
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Detection |
UV at 260nm,
0.01 AUFS |
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Sample |
TAKARA ras Gene
Probe set |
G910620C
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蛋白質(zhì)分析
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1. Ribonuclease
A (核糖核酸酶A)
2. Cytochrome C (細胞色素C)
3. Lysozyme (溶菌酶)
4. Myoglobin (肌紅蛋白) |
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Column |
YMC-Pack C4-AP
(5μm，300A) 150x4.6mm I.D. |
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Eluent |
A) acetonitrile/water/TFA (5/95/0.1); B) acetonitrile/water/TFA
(60/40/0.1)
30-90%B (0-20min，linear); 90%B (20-25min) |
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Flow rate |
1.0 mL/min |
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Temp |
37℃ |
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Detection |
UV at 220nm,
0.32 AUFS |
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Injection |
16 μL (0.16~0.33
mg/ml) |
B931023A
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上次更新時間：2010年06月07日
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