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Pro C18 RS |
產(chǎn)品規(guī)格 |
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YMC-Pack Pro C18 RS液相色譜柱的應(yīng)用實(shí)例
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YMC-Pack Pro C18 RS液相色譜柱對(duì)亞麻酸甲酯衍生物的分離
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Pro C18 RS
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Pro C18
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1. Methyl Linolenate (亞麻酸甲酯)
2. Methyl-γ-Linolenate (γ-亞麻酸甲酯) |
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Column |
YMC-Pack Pro C18 RS, 150x4.6mmI.D. |
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Eluent |
acetonitrile/THF/water (35/30/35) |
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Flow rate |
1.0 mL/min |
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Temp |
37℃ |
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Detection |
UV at 210 nm |
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左圖顯示了三種類(lèi)型的Pro色譜柱和一種競(jìng)爭(zhēng)產(chǎn)品在分離亞麻酸甲酯衍生物的比較，Pro
C18 RS可以很敏銳檢測(cè)到疏水性的不同，這一特點(diǎn)使其在對(duì)不飽和化合物的位置異構(gòu)體的分離非常有用。
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Hydrosphere C18
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Brand D4
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YMC-Pack Pro C18 RS液相色譜柱對(duì)氯代苯乙酮衍生物的分離
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1. o-Chloroacetophenone (鄰-氯代苯乙酮)
2. p-Chloroacetophenone (對(duì)-氯代苯乙酮)
3. m-Chloroacetophenone 間-氯代苯乙酮) |
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Column |
YMC-Pack Pro C18 RS, 5µm, 80Å, 150x4.6mmI.D. |
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Eluent |
acetonitrile/water/acetic acid (30/70/1) |
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Flow rate |
1.0 mL/min |
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Temp |
37℃ |
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Detection |
UV at 230 nm |
J011026B
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YMC-Pack Pro C18 RS液相色譜柱對(duì)氯酚衍生物的分離
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1. o-Chloroacetophenone (鄰-氯酚)
2. p-Chloroacetophenone (對(duì)-氯酚)
3. m-Chloroacetophenone (間-氯酚) |
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Column |
YMC-Pack Pro C18 RS, 5µm, 80Å, 150x4.6mmI.D. |
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Eluent |
acetonitrile/water/acetic acid (30/70/1) |
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Flow rate |
1.0 mL/min |
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Temp |
37℃ |
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Detection |
UV at 230 nm |
J011113D
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YMC-Pack Pro C18 RS液相色譜柱對(duì)堿性化合物的分離
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YMC-Pack Pro C18 RS
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Brand 3
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Column |
YMC-Pack Pro C18 RS, 5µm, 80Å, 150x4.6mmI.D. |
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Eluent |
20mM KH2PO4-K2HPO4(pH 6.9)/methanol (25/75) |
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Flow rate |
1.0 mL/min |
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Temp |
37℃ |
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Detection |
UV at 254 nm |
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1. Desipramine (去郁敏)
2. Diphenyl(I.S.) (聯(lián)苯)
3. Imipramine (丙咪嗪、米帕明)
4. Amitriptyline (阿米替林) |
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阿米替林是一種堿性化合物，在通常的一些硅膠基柱上均有嚴(yán)重的拖尾，上圖顯示了Pro
C18 RS色譜柱與競(jìng)爭(zhēng)產(chǎn)品耐酸色譜柱的比較，Pro C18 RS表現(xiàn)出優(yōu)良的色譜分離峰和無(wú)拖尾的現(xiàn)象。
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YMC-Pack Pro C18 RS液相色譜柱在LC/MS中對(duì)多肽的分離
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Column |
YMC-Pack Pro C18 RS, 5µm, 80Å, 150x4.6mmI.D. |
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Eluent |
A) water/TFA (100/0.01)
B) acetonitrile/TFA (100/0.01)
10-35%B(0-15min) |
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Flow rate |
0.2 mL/min |
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Temp |
37℃ |
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Detection |
ESI positve-mode |
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1.[Arg8]-Vasopressin (精氨酸8-后葉加壓素)
2.Bradykinin (緩激肽)
3.AngiotensinⅡ (血管緊張素Ⅱ)
4.Oxytocin (催產(chǎn)素、縮宮素)
5.AngiotensinⅠ (血管緊張素Ⅰ)
6.Leu-Enkephalin (亮氨酸-腦啡肽) |
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YMC-Pack Pro C18 RS液相色譜柱在低濃度的TFA流動(dòng)相中具有優(yōu)良的分離和色譜峰，其特別適合于LC/MS中應(yīng)用。
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