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德國(guó)耶拿-快速PCR試劑盒
Analytikjena Reagents for rapidPCR
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如果采用rapidPCR操作,就需要特殊的rapidPCR聚合酶,德國(guó)耶拿公司生命科學(xué)部除提供最優(yōu)化試劑盒外,還提供適配的聚合酶,以實(shí)現(xiàn)方便和可重復(fù)性的結(jié)果。
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SpeedTaq DNA Polymerase
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The SpeedTaq DNA Polymerase is specially optimized for extreme fast PCR. Extension times are reduced to a minimum due to a synthesizing speed of 200 bp/sec, what in return dramatically reduces the total reaction time. The SpeedTaq is a mixture of an ultrapure Taq Polymerase with a second thermal stable polymerase. This combination ensures utmost purity, reproducibility and processivity. Thus it is optimal for amplifications running on rapid thermal cyclers, as the SpeedCycler family from Analytik Jena | bio solutions.
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Concentration*: 5 U/µl |
Enzyme storage buffer: 20 mM Tris-HCl pH 8.0, 100 mM KCl, 1 mM DTT, 0.1 mM EDTA, 0.5% Tween 20, 0.5% Nonidet P40, 50% Glycerol |
PCR Buffer: 10x SpeedAmp Buffer (1 ml), buffer type has to be determined in advance using SpeedTaq Optimization Set (Order-No.: 845-EZ-1110100) MgCl2 is already included in the selected buffer, no addition necessary |
Store: at –20°C, avoid frequent thawing and freezing |
產(chǎn)品編號(hào)
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產(chǎn)品描述
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845-EZ-5000500 |
SpeedTaq DNA Polymerase, 500 units |
* One unit is defined as the amount of enzyme required to catalyze the incorporation of 10 nmol of dNTPs into a polynucleotide fraction in 30 minutes at 70°C.
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