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2025年7月12日 星期六
 

德國耶拿公司產(chǎn)品

基因擴(kuò)增儀(PCR)
實(shí)時熒光定量PCR儀
電泳和轉(zhuǎn)印系統(tǒng)
  聚酰胺凝膠電泳
瓊脂糖凝膠電泳
脈沖場電泳系統(tǒng)
溫度梯度電泳系統(tǒng)
手工核酸測序電泳
電泳電源系統(tǒng)
印跡及轉(zhuǎn)印儀
凝膠電泳耗材
印跡及轉(zhuǎn)印儀(Bloting)
  Fastblot B33/B34
Fastblot B43/B44
Fastblot B64
Tankblot電轉(zhuǎn)印儀
Tankblot Eco-Mini
Tankblot Eco-Maxi
真空轉(zhuǎn)印儀/系統(tǒng)
毛細(xì)管轉(zhuǎn)印儀
斑點(diǎn)印跡轉(zhuǎn)印儀
雜交印跡轉(zhuǎn)印儀
可過濾轉(zhuǎn)印儀
凝膠成像系統(tǒng)
生物分析系統(tǒng)
核酸提取純化系統(tǒng)
液體樣品處理系統(tǒng)
振蕩混合和快速勻漿
紫外可見分光光度計
MobiLab移動實(shí)驗(yàn)室
耶拿-試劑/耗材/配件
耶拿-分析和診斷試劑

相關(guān)產(chǎn)品鏈接

 
 

耶拿 - 印跡(Blotting)系列產(chǎn)品

analytikjena Series Products of Blotting

Transfer of proteins and nucleic acids on to the surface of immobilising membranes

  • Protein blotting
  • Nucleic acid blotting
  • Sample concentration/immobilisation
Blotting is a standard method for the transfer of molecules on to the surface of a membrane. Additionally blotting is an intermediate step in protein sequencing and an elution method for subsequent analysis.
Electro-blotting is an important method to transfer mainly proteins from polyacrylamide gels on to nitrocellulose or other carrier membranes (Western blotting). Tank blotting is done in vertical buffer tanks with coiled platinum wire electrodes on two sides. For this technique, the gel and blotting membrane are clamped in a frame between filter paper and fiber pads. Typical transfer time is over night. Semi-dry blotting is done between two horizontal plate electrodes and offers fast as well as homogeneous transfer. In contrast to tank blotting only little transfer buffer is required and transfer times are very short (several minutes). Additionally, with semi-dry blotting discontinuous buffer systems can be used to gently blot smaller proteins or to transfer protein mixtures of very different sizes more evenly.
Transfer of nucleic acids (Southern / Northern blotting) is traditionally done by capillary blotting, a time-consuming procedure that usually takes up to 12 hours (over night) to complete. Therefore vacuum blotting is mostly used instead of capillary blotting. With vacuum blotting transfer times can be reduced to 15 - 60 minutes.
Dot and Slot blotting techniques are used for concentration and immobilisation of samples (proteins and nucleic acids) on to a membrane with the use of a vacuum source.
Fastblot B33/B34(快速半干) Fastblot B43/B44(快速半干)
Fastblot B64(快速半干) Tankblot電轉(zhuǎn)印儀(蛋白質(zhì)濕法)
Tankblot Eco-Mini(蛋白質(zhì)濕法) Tankblot Eco-Maxi(蛋白質(zhì)濕法)
真空轉(zhuǎn)印儀/系統(tǒng)(Vacu-Blot) 毛細(xì)管轉(zhuǎn)印儀
斑點(diǎn)印跡轉(zhuǎn)印儀(Dot Blot) 雜交印跡轉(zhuǎn)印儀
可過濾轉(zhuǎn)印儀  
   
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