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產(chǎn)品簡介
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真正的快速解決方案 |
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The iQ-Check system takes full advantage of real-time
PCR to provide a faster reliable solution in food pathogen
testing. A complete range of kits are available for
qualitative detection of major food pathogens in all
food and environmental samples. Due to the high sensitivity
and specificity of real-time PCR, a single overnight
enrichment is sufficient to obtain results equivalent
to or better than ISO or other reference methods. |
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iQ-Check的原理 |
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Genes targeted are specific: for example, the gene
involved in the invasive bacterial process (iagA) for
detecting Salmonella spp. and the gene involved in the
listeriolysine virulence process (hlyA) for detecting
Listeria monocytogenes. Detection of the fluorescent
signal of these amplified sequences is an indication
of the presence of the pathogen in the sample being
tested. In a multiplex assay, each PCR well has an internal
control DNA, which is amplified at the same time as
the target DNA, but detected by a different probe and
fluorophore. |
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Maximum Flexibility |
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Developed as a flexible, open system, the iQ-Check
kits can be used with different Bio-Rad real-time PCR
instruments. A single sample or as many as 94 samples
can be run on either smaller instruments or higher throughput
instruments, all to fit a laboratory's needs and throughput.
Both a 96 and 48-well instrument are available, with
the ability to run up to 4 instruments from a single
computer at the same time. |
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Optimized Cost and Work Flow |
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With a time-to-result of 24 hrs or less, it is now
possible to optimize cost and reactivity, improving
HACCP procedures and overall productivity. Innovative
media, reagents and leading technology provide a complete
solution for the most efficient control of food products
and the industrial environment. |
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iQ-Check系統(tǒng)采用實(shí)時(shí)熒光定量PCR方法所有的的優(yōu)點(diǎn)，為水中病原微生物提供快速和可靠地檢測(cè)解決方案。iQ-Check實(shí)時(shí)熒光定量PCR試劑盒為所有環(huán)境水中的軍團(tuán)菌(Legionella)和嗜肺型軍團(tuán)菌(Legionella
Pneumophila)定性和定量檢測(cè)試劑盒。 |
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iQ-Check實(shí)時(shí)熒光定量PCR試劑盒是基于基因?qū)W方法用于食品病原微生物和水中病原微生物檢測(cè)的突破，iQ-Check實(shí)時(shí)熒光定量PCR試劑盒采用聚合酶鏈?zhǔn)椒磻?yīng)(PCR)和實(shí)時(shí)檢測(cè)和定量技術(shù)相結(jié)合，iQ-Check軍團(tuán)菌實(shí)時(shí)熒光定量PCR試劑盒包括：DNA引物和特異生物的熒光分子探針。如果水樣中存在軍團(tuán)菌和嗜肺型軍團(tuán)菌，DNA得以擴(kuò)增和熒光強(qiáng)度增加，其被iCycler
iQ系統(tǒng)實(shí)時(shí)檢測(cè)和記錄。在每個(gè)反應(yīng)管內(nèi)存在的內(nèi)標(biāo)通過檢測(cè)抑制因子的存在和陰性結(jié)果可靠性的確認(rèn)來評(píng)估每個(gè)擴(kuò)增反應(yīng)的效率。每個(gè)反應(yīng)包含有兩種熒光染料，用作目標(biāo)DNA和內(nèi)標(biāo)的標(biāo)記探針，包括一套定量標(biāo)準(zhǔn)的以保證軍團(tuán)菌和嗜肺型軍團(tuán)菌的實(shí)時(shí)定量檢測(cè)(推薦采用Chromo4型四色實(shí)時(shí)熒光定量PCR儀)。 |
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iQ-Check的特點(diǎn)
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Faster Results |
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Test results within hours after overnight enrichment |
| Colony confirmation with validated chromogenic agar |
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Easy To Use |
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Simple DNA extraction |
| Automated amplification and detection |
| Run multiple pathogen tests at the same time |
| Ready-to-use reagents, including extraction reagents |
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Minimal Risk |
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Positive, negative and internal PCR inhibition controls |
| Specific primers and probles |
| No post-PCR steps for minimal contamination risk |
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A Complete Solution |
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Standardized Bio-Rad reagents: |
| - Enrichment media |
| - Real-time PCR iQ-Check kits |
| - Chromogenic agars |
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Validated Bio-Rad equipment and software: |
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- Real-time PCR detection systems |
| - Automated data interpretation and traceability |
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iQ-Check試劑盒產(chǎn)品信息
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產(chǎn)品編號(hào) |
產(chǎn)品描述 |
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78123 |
Kit for the detection of Salmonella spp.
iQ-Check沙門氏菌屬檢測(cè)試劑盒，96個(gè)測(cè)試/盒 |
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78124 |
Kit for the detection of Listeria monocytogenes
iQ-Check單增李斯特菌檢測(cè)試劑盒，96個(gè)測(cè)試/盒 |
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78113 |
Kit for the detection of Listeria spp.
iQ-Check李斯特菌屬檢測(cè)試劑盒，96個(gè)測(cè)試/盒 |
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78114 |
Kit for the detection of E.coli O157:H7
iQ-Check大腸桿菌O157:H7檢測(cè)試劑盒，96個(gè)測(cè)試/盒 |
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